Fluorescence Microscopy
Example 2

To the right are three JPEG images of bovine endothelial cells stained with fluorescent dies, courtesy of Dr. M. V. Partasarathy, Cornel University, 2002. The green structures are actin filaments, the red structures are green, and the nuclei are stained blue. The scale bar is 40 microns.

The raw image (top) (Raw_scale 40 microns) can be considerably improved by normalizing image intensities (bottom image) (Normalized_scale40 microns). The normalization feature is commonly included in most image acquisition software. However, when you use the normalization method to boost the intensity of a particular cell that has a low signal, you often wind up with over-saturated signal in other areas (see white arrows in  Normalized_scale40 microns).

On the other hand processing the raw image with Lucis Pro in Split Channel mode (middle image) allows you to intensify low signal color channel in one region of the image without over-saturating  normal signal in another region. For example, note the overall improved red channel signal in Lucis scale 40 microns, and compare the cells indicated with white arrow in Normalized_scale40 microns with same cells in Lucis scale 40 microns. You will notice that  the red channel  in the cells indicated are over saturated in the  Normalized image.

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